Adam, Maryanne, Brian and Sheralyn Stephenson, of Bristol, died when their rental car was hit by a pickup truck.
Titusville Police Department confirmed all four members of the Stephenson family were pronounced dead at the scene.
The force said the Stephensons’ white Mitsubishi sedan, which was being driven west on State Road 405 by 30-year-old Adam Stephenson, turned left at the junction with Sisson Road where it was struck by a “heavy-duty” black Ford F-250 pickup.
The other occupants were Maryanne Stephenson, 29, Brian Stephenson, 66, and Sheralyn Stephenson, 56.
Police investigators believe the family had just left the Kennedy Space Center and were returning to their rental home in Davenport, around 85 miles away.
The pickup driver suffered non life-threatening injuries.
A Foreign Office spokesperson said: "We are supporting the family of four British people who have died in Florida.
"Our thoughts are with them at this deeply difficult time."
