The European Union Aviation Safety Agency (EASA) and European Centre for Disease Prevention and Control (ECDC), which issued the update on Wednesday (11 May), said the new rules will come into effect on 16 May.
Despite the relaxation, the organisations noted a face mask is "still one of the best protections" against Covid-19 and warned the rules may vary between airlines.
EASA executive director Patrick Ky said it was a "relief to all of us" health safety measures can start to be relaxed.
"For many passengers, and also aircrew members, there is a strong desire for masks to no longer be a mandatory part of air travel," he added. "We are now at the start of that process.
"Passengers should continue to comply with the requirements of their airline and, where preventive measures are optional, make responsible decisions and respect the choice of other passengers."
