The company’s Blue World Project is scheduled for completion in 2018 and will expand the killer whale habitat at a cost of $100 million.
The last-minute amendment would ban the breeding – including via artificial insemination – at the park, but not at SeaWorld’s other facility, according to the Associated Press.
In a statement SeaWorld expressed disappointment at the decision.
“Breeding is a natural, fundamental and important part of an animal’s life and depriving a social animal of the right to reproduce is inhumane,” SeaWorld said.
It is not clear whether SeaWorld will appeal the decision.
The vote follows an all-day hearing where people argued for and against the expansion.
People for the Ethical Treatment of Animals (Peta) lawyer Jared Goodman said: “These 11 orcas would be the last 11 orcas there.”