The allegations were aired during a BBC Panorama investigation, which was broadcast earlier this week. A third woman told the BBC they were subject to a non-consensual sex act while taking part in filming the show.
Tui struck up a partnership with the UK edition of the show in 2024; this was extended last year to cover both Married at First Sight UK and Married at First Sight Australia content.
After Panorama aired, Tui initially said it had paused its sponsorship activity while it reviewed the content of the Panorama investigation and "the serious allegations" it raised.
A spokesperson for Tui on Wednesday (20 May) subsequently confirmed to TTG the operator had ended the partnership.
"Following the broadcast of the Panorama programme and discussion with Channel 4, we have taken the decision to end our sponsorship of Married at First Sight," said the spokesperson.
Channel 4 has commissioned an external review of welfare on the show, the UK edition of which is produced by independent production company CPL.
Married at First Sight – better known just as MAFS – is billed as a "social experiment" which sees single people marry strangers they meet for the first time at a mock wedding ceremony.
The show them documents their honeymoons, their lives together and how they navigate their new relationships. All three women told the BBC they spoke out because they felt they should have been better protected.